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Rev. argent. microbiol ; 42(2): 98-101, abr.-jun. 2010. tab
Article in Spanish | LILACS | ID: lil-634651

ABSTRACT

Se evaluó la prevalencia serológica del virus de influenza mediante las pruebas de inhibición de la hemaglutinación (IHA) y ELISA para los subtipos H1N1 y H3N2 en 13 granjas porcinas de Argentina. Se compararon los resultados obtenidos mediante ambas pruebas en términos individuales y de establecimientos. La prevalencia individual por la técnica de IHA fue de 38,46% a 100% para H1 y de 7,69% a 100% para H3. Por la técnica de ELISA, la prevalencia individual fue de 2,33% a 6,9% para H1 y de 9,65% a 48% para H3. No se observaron diferencias significativas entre ambas técnicas a escala de granja (H1: p=0,20; H3: p=0,11). La concordancia entre las pruebas fue nula al tomar como unidad de referencia el animal (H1: 0,005; H3: 0,070), mientras que en términos de establecimiento fue escasa (H1: 0,350; H3: 0,235). Considerando la alta prevalencia individual obtenida por la prueba de IHA y la alta sensibilidad de esta técnica, se podría sugerir que en las poblaciones porcinas de la Argentina circularon cepas virales humanas o cepas porcinas con gran proximidad filogenética a las utilizadas en este estudio desde el año 2002.


The seroprevalence of the Influenza virus against H1N1 and H3N2 was determined by the hemagglutination-inhibition test (HI) and a commercial swine influenza ELISA kit, in 13 Argentinean swine herds. The results of within-herd and between-herd prevalence obtained by both tests were statistically correlated. The within-herd prevalence observed by the HI test varied from 38.46 to 100% against H1 and 7.69 to 100% for H3. When the within-herd prevalence was measured with the ELISA test, it varied from 2.33 to 6.9% for H1 and 9.65 to 48% for H3. No statistical differences were observed at herd level between HI and ELISA (H1: p = 0. 20; H3: p=0.11). No agreement between HI and ELISA detected prevalence was observed when the within-herd prevalence was compared (H1: 0.005; H3: 0.070), while the agreement at herd level was considered poor (H1: 0,350; H3: 0,235). The high within-herd prevalence values observed with the HI test and the high sensibility of this test might show that human strains or swine strains phylogenetically closely related to the humans strains used in the HI test in this study have been affecting the swine population since 2002.


Subject(s)
Animals , Humans , Antibodies, Viral/blood , Enzyme-Linked Immunosorbent Assay/veterinary , Hemagglutination Inhibition Tests/veterinary , Influenza A virus/isolation & purification , Orthomyxoviridae Infections/veterinary , Sus scrofa/virology , Swine Diseases/epidemiology , Argentina/epidemiology , Disease Reservoirs/veterinary , Influenza A Virus, H1N1 Subtype/immunology , Influenza A Virus, H1N1 Subtype/isolation & purification , Influenza A virus/classification , Influenza A virus/immunology , Influenza, Human/epidemiology , Influenza, Human/virology , Orthomyxoviridae Infections/diagnosis , Orthomyxoviridae Infections/epidemiology , Orthomyxoviridae Infections/virology , Predictive Value of Tests , Seasons , Sensitivity and Specificity , Seroepidemiologic Studies , Swine Diseases/diagnosis , Swine Diseases/virology , Swine/virology
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